Ph. D Degree — Ciencia sem Fronteiras. Background. It is well known that antimicrobial resistance is transmitted through the food chain but what is the magnitude and importance for human disease is difficult to quantify due the complexity of this. Antimicrobial resistance can be transmitted from non- pathogenic antimicrobial resistant bacteria, zoonotic bacteria or as mobile genetic element that can be exchanged in the human gut to the host adapted intestinal flora or to human specific pathogens resident in the host. The magnitude and the mechanisms that are of most importance for transfer to the consumers are unknown since very few quantitative data exist.
I am getting ready to set up a home business, since I am not quite ready to retire and I am already collecting a pension from the state of North Carolina. I started working for the Department of Transportation when I was fresh. California NanoSystems Institute - The California NanoSystems Institute (CNSI) is a research center at UCLA whose mission is to encourage university collaboration with industry and to enable the rapid commercialization of. The emission of ammonia (NH 3) from agricultural systems is a major environmental problem. Most of the NH 3 emission comes from animal production, especially from liquid manure (mixture of urine and feces. Today presence of antimicrobial resistance is mostly defined using indicator organism like enterococci and Escherichia coli (E. But what is the magnitude (the bulk) of antimicrobial resistance being exposed to consumers and what is the most important source in the food chain. As a generic example a quantitative analysis for indicators (E. Preliminary data on tetracycline resistance in indicator bacteria isolated from pigs suggest that the resistance pool entering the food chain at harvest can be excessive. However, it seem that the food processing line reduce and dilute this from harvest to consumer (retail). In contrast, development of cross resistance due to biocides / disinfectants, metals and bacterial growth can amplify the amount of resistance finally exposed to the consumer. As for the vancomycin resistant enterococci (VRE) they were observed at high prevalence in the animal reservoir in the 9. Denmark and then the European Union in the 9. It has however not been possible to identify a clear link between the human pathogenic VRE types in the animal reservoir, since host specific clones have introduced barriers for direct transmission of VRE while evidence for identical genes are present. Prevalence of VRE have been drastically reduced in the animal reservoir (they are still present) lowering the exposure of the consumer. Furthermore recent studies with methicillin resistant Staphylococcus aureus (MRSA) have identified specific clones related to the production animals which are not (yet) of high importance in the human reservoir. Do special bacterial clones play a significant role in transmission of resistance both from the food chain and the environmental reservoir to the human reservoir? A hypothesis, which may explain the apparent limited transmission of antimicrobial resistance from animals to human pathogenic bacteria could be restriction of survival and growth of the donor (coming from an animal host going the food production facilities ) when entering the human host. Growth is essential for effective horizontal transfer of antimicrobial resistance. Thus only bacteria (species or clones) that are capable of persisting (colonizing and growing) in both the animal and the human reservoir but also survive through the food production facilities may be the important drivers of exchange of antimicrobial resistance genes. If the species barrier (examples are seen in E. The latter will be candidates for vectors, which we claim could enable transfer antimicrobial resistance from the animal reservoir to the human reservoir. The collection of members of E. An element could be to access the combined impact of ABR entering the processing line and the fate during processing. Objective. By isolation of enterococci, E. Full genome sequencing will give information of clonal relationship, presence of mobile genetic elements and antimicrobial resistance genes that can be used to quantify the prevalence of both species and resistance genes. Using Q- PCR quantifying data will be obtained. Laboratory and animal experiments using host specific clones for the human and animal reservoir will be set up to verify and understand the species barriers and their importance for antimicrobial transmission. Contact. Lars Bog. Human and Swine share Vancomycin- resistant Enterococcus faecium CC1. CC5 and Enterococcus faecalis CC2 clonal clusters harbouring Tn. Porcine and human community reservoirs of Enterococcus faecalis associated with infective endocarditis in humans, Denmark. Shuyu Wu, S., Dalsgaard, A., Hammerum, A. M., Porsbo, L. J. Prevalence and characterization of plasmids carrying sulfonamide resistance genes among Escherichia coli from pigs and human. Acta Veterinaria Scandinavica 2. Jakobsen, L., Spangholm, D. J., Pedersen, K., Jensen, L. B., Emborg, H. D., Agers. Broiler chickens, broiler, chicken meat, pigs and pork as sources of Ex. PEC related virulence genes and resistance in Escherichia coli isolates from community- dwelling humans and UTI patients. Wu, S.,Chouliara, E., Jensen, L. B. Evaluation of Petrifilm. TM Select E. Scand., 2. Agers. 2. 00. 8. Vancomycin- resistant Enterococcus faecalis isolates from a Danish patient and two healthy human volunteers are possibly related to isolates from imported turkey meat. Vieira, A., Wu,S., Jensen, L. B., Dalsgaard, A. Houe, H., Wegener, H. C., Wong, L. F. Using data on resistance prevalence per samples for antimicrobial resistance surveillance. Antimicrob. Agents Chemother 6. Download - Update. Star - Update. Star. Download the. free trial version below to get started. Double- click the downloaded file. Update. Star is compatible with Windows platforms. Update. Star has been tested to meet all of the technical requirements to be compatible with. Windows 1. 0, 8. 1, Windows 8, Windows 7, Windows Vista, Windows Server 2. Windows. XP, 3. 2 bit and 6. Simply double- click the downloaded file to install it. Update. Star Free and Update. Star Premium come with the same installer. Update. 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In the 6. 0's a standards committee now known as the Electronic Industries Association developed an interface to connect computer terminals to modems. Over the years this has been updated: the most commonly used version of the standard is RS2. C (sometimes known as EIA2. Jump to: navigation, search. RFC 2217 defines a protocol to transfer data from/to a serial device over TCP. Serial communication on pins TX/RX uses TTL logic levels (5V or 3.3V depending on the board). Don't connect these pins directly to an RS232 serial port; they operate. Hercules SETUP utility is useful serial port terminal (RS-485 or RS-232 terminal), UDP/IP terminal and TCP/IP Client Server terminal. It was created for HW group. RS2. 32. E. The standard defines the electrical and mechanical characteristics of the connection - including the function of the signals and handshake pins, the voltage levels and maximum bit rate. If RS2. 32 is a standard why can't I just use a standard lead to connect together two RS2. That's a good question. The answer is that the RS2. The standard was defined to connect computers to modems.
Free Serial Analyzer Features. Free Serial Port Analyzer is able to capture and decode monitored packets in real-time even under high traffic load.Any other use is outside of the standard. The authors of the standard had in mind the situation below. The standard defines how computers (it calls them Data Terminal Equipment or DTEs) connect to modems (it calls them Data Communication Equipment or DCEs). The standard says that computers should be fitted with a 2. D socket. The interconnecting lead between a computer and a modem should be simply pin. The main signals and their direction of flow are described below. It is important to note that a signal which is an output from a computer is an input to a modem and vice versa. This means that you can never tell from the signal name alone whether it is an input or an output from a particular piece of equipment. Also, instead of being a DCE device, a data acquisition device might be configured as DTE. In this case you need an adaptor or the RS2. When the PC is connected to a DTE instrument - called a null modem arrangement - some of the cable wires must cross over. It can be read by the computer to determine that the device is on line. It can be read by the device to determine that the computer is on line. The standard envisaged that when a computer wished to transmit data it would set its RTS. How Serial Ports Work. The local modem would then arbitrate with the distant modem for use of the telephone line. If it succeeded it would set CTS and the computer would transmit data. The distant modem would use its CTS to prevent any transmission by the distant computer. The pin connections used are: Pin Direction. Signal. 1 Input. DCD Data Carrier Detect. Input. RXD Received Data. Output. TXD Transmitted Data. Output DTR Data Terminal Ready. Signal Ground. 6 Input DSR Data Set Ready. Output RTS Request To Send. Input. CTS Clear To Send. Input RI Ring Indicator. Way Pin to 2. 5- Way Pin. DB9 DB2. 5 Signal 1 8 DCD Data Carrier Detect 2 3 RXD Received Data 3 2 TXD Transmitted Data 4 2. DTR Data Terminal Ready 5 7 Signal Ground 6 6 DSR Data Set Ready 7 4 RTS Request To Send 8 5 CTS Clear To Send 9 2. RI Ring Indicator. Null Modem Connections. Instead of being a DCE device (see above for details), a data acquisition device might be configured as DTE. In this case you need an adaptor or the RS2. When the PC is connected to a DTE instrument - called a null modem arrangement - some of the cable wires must cross over. For example, when there is no hardware handshaking in place the connections are as follows. DB9 DB9 Signal. 23. RXD Received Data - TXD Transmitted Data. TXD Transmitted Data - RXD Received Data. Signal Ground. You've plugged your instrument into your computer's COM. You suspect it is a problem with your. RS2. 32 communications. This provides many features which let you detect, and correct, communication errors. If you can't immediately obtain data with Com. Debug, follow these steps. Find Communication Details: Baud Rate etc. You will need to know the number of the com port into which you have plugged your device, the device baud rate, number of bits in the data byte and parity. Enter this information in Com. Debug. If you have information about the Stop Bits then use it, otherwise set them to 2. This will at worse slow the message down slightly. See Making Measurements through the COM Port for a detailed explanation of each of these settings. Tips on Using USB- to- Serial Converters gives more information. Send a message. If you can persuade your device to send a message, perhaps a power on message or a message that can be initiated by a button press, then do so. This will verify your signal connections. At this stage you should select the no handshake option when setting up the COM Port. If you still can't receive any data, you may have a wiring error. Use the Com. Debug program's COM Port Status window to check the state of the lines to and from the computer, and see points 3, 4 and 5 below. This loopback test works if your instrument is a DTE computer type with a 9- pin connector. Disconnect the RS2. COM port is working correctly. Check the Cabling. If you suspect a wiring error, you could try contacting. Sartorius devices, for example. RS2. 32 cable and you need to. If your instrument is a DTE computer type. Null Modem adaptor. Alternatively, you may want to try rewiring the. Try to establish, from your device's documentation, which signal wire carries its output data - connect this to the computer's RXD. The signal which inputs data to your device should be connected to the computer's TXD. Don't rely on the signal names, remember the signals can be either inputs or outputs depesnding on whether your device is a computer or modem type. It's not unusual for Instrument Manuals to neglect this vital information, but you may be able to get a clue from other signals. For instance if the manual says that DTR is an output then the instrument should be a computer type. If on the other hand it says that DSR is an output then it should be a modem type. In fact if you know the direction of any one of the signals you should be able to deduce the rest. Be careful when doing this: we have encountered manufacturers who change the names of the data signals when dealing with modem types - after all it must be confusing to tell a user that Transmitted Data is an input. If the signals are correctly named then a computer to modem link connects TXD to TXD and RXD to RXD. A computer to computer link (more common when dealing with instruments) connects TXD to RXD and RXD to TXD. This crossed arrangement is sometimes called a Null Modem connection, and buying an adaptor may solve your wiring problems. The output signal will be at a definite negative voltage, properly - 1. Volts or more. The input line will be close to 0. V. The only time this strategy will fail is if your device is of the multidrop variety. These only drive the output line for the duration of the transmitted message. Set Hardware Handshaking or Flow Control. If you are confident that the signal wires are properly connected, but you still can't retrieve a message from your instrument, you may need to tie the handshake lines. Firstly the computer can prevent the device from sending data when it is not able to receive it. Secondly the device can prevent the computer from sending data when it is not ready for it. The fact that your device comes equipped with inputs and outputs that can be used for handshaking is no guarantee that handshaking is needed. The signals are often provided simply because the processor used in the device provides them, so the manufacturer feels he may as well put them on the plug. It is usually best to start with the intention of tieing any potential handshake lines to fixed voltages so that they do not affect operation. In fact many manufacturers add tieing resistors to handshake lines so that if you do not want to use them you simply make no connection. Well one possibility is that the computer misses part of a message because its input buffer overflows. In COMIML the buffers are 3. The other possibility is that the device misses part of a message sent by the computer. This will presumably cause the device not to operate as desired. If you do decide that handshaking must be used then COMIML uses DTR / CTS handshaking. This means that the computer uses its DTR output to indicate when it is able to receive data and its CTS input can be controlled by the device to prevent transmission of data from the computer. Once you select the hardware handshake option the state of the CTS input to the computer becomes important. When hardware handshake is not selected the CTS line state is ignored, the DTR output however is maintained permanently high so you can use it to tie unused inputs on your device. If your instrument needs DTR to be low, see below. Set Software Handshaking or Flow Control. Xon \ Xoff Handshaking is a software protocol that is often used to control data flow. Supposing that the computer were sending data to a device which could accept no more data for the time being - the device would send the single Xoff character to the computer which would stop sending data until it received an Xon character to restart transmission. The same arrangements would apply for the reverse direction of data flow. If your device requires this type of handshaking then simply select it in Com. Debug. This shows you the state of the computer's COM port. If you are not receiving replies from the instrument this may help you identify the problem. Red indicates true or high, green false or low. CTS - Clear To Send. If you are using Hardware Flow Control in your COM Settings, CTS must be true. If it is true you may have a wiring error. If it is true you may have a wiring error. RI - Ring Indicator. Should have no effect on data flow and will normally be false. If it is true you may have a wiring error. COM Output Lines. You can control the state of these lines - check your instrument's Manual for details of the settings it expects. It lets the instrument check that the computer is on line. Also, the Pub. Sub pattern is great because of its flexibility. You can build master/slave applications, device to device, etc. C library The C library is very simple to add on any new device as long as you have a decent UART library on it. You just have to instanciate a data structure called TM. The Partnership Agreement Template can be divided into three parts. The first part is the main body of the partnership agreement, which contains seven core sections. 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